Neurobiology Laboratory - BIO365L

Required Reading

• Course Syllabus
• The Axon Guide
• Module 1
  • Lab Manual for Module 1
• Intro to Light Microscopy and Patch Clamp
• Chabbert et al. (2002). An article on the Ih current and the HCN channel
• Module 2
  • Lab Manual for Module 2
  • An excellent review of action potential mechanisms
  • Review of Ca++ activated K+ channels
  • Role of the M-current in the medium AHP
  • Role of BK channels in spike patterning
  • Connection betweeen learning and the fast AHP
  • Dopamine modulates the slow AHP
• Module 3
  • Lab Manual for Module 3
  • Chen et al. (1999). Induction requirements for LTP.
  • Krasteniakov et al. (2004) Are action potentials necessary for LTP?

Lecture Notes

• Week 1 - Class intro and passive properties. Capacitance compensation, bridge balance
• Week 2 - Patch Clamp technique
• Week 3 - Basics of light microscopy
• Module 2 Lecture - Active properties of neurons
• Module 3 Lecture - Synaptic transmission

Checklists and Protocols

• Training Exercise 1
• Training Exercise 2
• Patch Clamp Guide
• Student Cleanup Checklist
• Instructor Cleanup Checklist
• Saline recipes